FastQCFastQC Report
Thu 25 May 2017
62365115_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename62365115_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57043848
Sequences flagged as poor quality0
Sequence length35-110
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG54200.049.326949
ATCGGAA86850.031.7473581
GAGCGTC89200.030.3772688
CGCCGTA42100.029.2526656-57
TCGGAAG99550.027.993092
GCGTCGT54200.025.51692410-11
AGAGCGT113550.023.9578387
CGTCGTG58500.022.5866910-11
CGGAAGA124800.022.4286233
TCGCCGT57650.022.20966556-57
GCCGTAT65950.019.5692758-59
AAGAGCG144450.019.438686
GTCGCCG72700.018.4262654-55
GTCGTGT79850.017.28743712-13
TGGTCGC79800.017.110152-53
GTGGTCG85100.016.98117352-53
GGTCGCC83800.016.81397654-55
TCGTGTA81500.016.3697112-13
CCGTATC76700.016.2566958-59
CGTATCA84650.015.36819260-61
Produced by FastQC (version 0.11.5)